Androlog Mail
{Regarding Dr. Meacham's question about 100% non-motile sperm:}
I have seen a couple of cases like this with 2 different causes.
Do you know if this gentleman has Kartagener's syndrome (I've also heard it
called immotile cilia syndrome)? I would do a HOS test and an
eosin-nigrosin stain. Both tests are quite simple. You can perform ICSI
using the HOS test. It is technically difficult to pick up curled sperm
which have been exposed to HOS solution. But you can pick the sperm up from
culture media, transfer your needle into a drop of HOS solution, hold the
sperm near the opening of the needle and allow the HOS solution to diffuse
into the needle and you can watch the sperm start to curl inside the ICSI
needle. As soon as you see the sperm tail curl, you can move to a PVP drop,
break the tail and perform the ICSI. This particular couple had twins using
this technique.
In the other case, the individual simply didn't follow proper collection
technique and had been using Lubriderm lotion. As soon as he collected
without the Lubriderm, his motility was in the normal range.
If you can be sure that you have live/viable sperm, I don't know of any
reason to perform a biopsy but I'd be interested in others thoughts.
Dawn A. Kelk, PhD
Assistant Professor and Director IVF & Andrology Laboratories
Department of Ob/Gyn
University of Colorado Health Sciences Center
4200 E. 9th Av., Box E198
Denver, Colorado 80262
Email: Dawn.Kelk@uchsc.edu <mailto:Dawn.Kelk@uchsc.edu>
Office: Phone 303-372-1440, Fax 303-372-1499
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