Androlog Mail:
RE: Dr. Sid Sarkar's query on mixing two semen samples for the same IUI:
In the early 80's, we used to "pool" semen samples to increase the volume
of the initial sample, before aliquoting them in to different control and
test groups, to run experiments on human sperm using the hamster
penetration assay. The semen would be pooled hardly for 5 minutes, before
being washed by centrifugation followed by resuspension in culture media.
The same concentration of motile sperm was added to each dish. A typical
result would look like this:
Hamster egg penetration with sample A: 70%
with sample B: 65%
with sample A and B mixed in equal proportions: 40%
The results were pretty consistent. I naturally came to the conclusion
that some how the samples were creating a detrimental effect for each
other. I think that later a paper came out showing that it was the seminal
plasma part of some semen samples that created a negative effect on the
penetration rate of other samples. I left mainstream research and never
followed up on this observation. I seem to recall that some one from
England observed the same phenomenon and included this observation in a
book chapter. I agree that IUI should be attempted with one sample at a
time.
Rita Basuray, PhD, HCLD
Washington University, Dept. of OB/GYN, St. Louis
basurayr@msnotes.wustl.edu
314-286-2440 (FAX 314-286-2455)
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