Androlog Mail:
Regarding seminal fructose testing, a very simple yet reliable qualitative
fructose test has been around for years. The procedure is as follows (Keel,
Handbook of the Laboratory Diagnosis and Treatment of Infertility, CRC Press,
1990):
1. Add 50 mg resorcinol to 33 ml concentrated HCl. Dilute to 100 with
distilled H2O. Reagent may be stored in amber bottle, at 4C, for up to one
year.
2. Mix 0.5 ml well-mixed fresh semen with 5.0 ml resorcinol reagent.
3. For controls, mix 0.5 ml H2O (negative) or 0.5 ml of 2 mg/ml fructose
standard in H2O (positive) with 5.0 ml reagent.
4. Thoroughly mix and bring to a boil by placing tubes into a beaker of
boiling water.
5. An orange-red color will appear within 60 sec. after boiling if fructose is
present. If fructose is absent, the solution will remain colorless.
There are, of course, some very good quantitative methods for measuring
fructose in semen. However, it has always been my contention that a "presence
or absence" result is just as meaningful, and a great deal easier to
determine!! I hope this helps.
Brooks A. Keel, Ph.D., HCLD
bkeel@kumc.edu
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